X-ray crystallography |
VLP binding to saliva-coated microtiter plates |
Titration calorimetry |
Thin-layer chromatography (TLC) |
Surface plasmon resonance (SPR) |
Solid-phase binding assay |
Site-specific mutations in fedF introduced by overlap PCR |
SDS-PAGE electrophoresis |
Recombinant lectin expression in E. coli strain Top10 |
Recombinant lectin expression in E. coli strain BL21 |
Radiolabeled fungal cells |
Radiolabeled bacteria |
Radiolabeled antibodies |
Radio labeled bacteria |
Photomicroscopy |
Overlay of thin-layer chromatography (TLC) plates with S-labeled bacteria |
Nuclear magnetic resonance (NMR) |
Mutagenesis studies |
Mucin binding assay |
Monoclonal antibody binding to ligands |
Monoclonal antibody (MAb) blocking of Gp120-GalCer binding |
Molecular docking program HADDOCK |
Model with Coot |
Microtiter well assay |
Microscopic examination of bacteria bound to human cells |
Microscale thermophoresis (MST) |
Matrix-assisted laser desorption |
MAbs specific to histo-blood group antigens |
MAD |
Isothermal titration calorimetry |
Ionization time-of-flight (MALDI) MS |
Inhibition of infection by anti-GalCer monoclonal anti-bodies |
Inhibition of binding of FITC-labeled bacteria to gastric mucosa |
Immunolabeling of C. pylori for examination by electron microscopy |
Immunofluorescence labeling |
Immunofluorescence assay and confocal microscopy |
Immobilized virus bound to labeled synthetic glycopolymers |
High performance thin layer chromatography (HPthin-layer chromatography (TLC)) binding assay |
Hemagglutination assay |
Hemagglutination (indo-beta-galactosidase-treated human erythrocyte) |
HA inhibition assay |
Gp120 binding |
Glycolipid analysis |
Glycan microarray analysis |
Glycan complex |
GL isolated and characterized by FAB and EI MS and proton NMR |
Fused-gene expression |
Fluorescence immunocytochemistry |
Flow cytometry |
ELISA, removal of N-linked oligosaccharides |